John Terry and Ashley Cole are expected to be fit for Chelsea's game at QPR on Saturday and are set to take part in the pre-match handshake.
Terry and Cole have been in a race against time to recover from ankle injuries for the west London derby at Loftus Road in the Premier League.
But they have trained without incident for the past three days and are set for a reunion with Anton Ferdinand, who Terry was cleared in court of of racially abusing back in July.
"They've recovered, had a couple of days training and we expect them to be available for tomorrow," Di Matteo said.
"We're going to respect the rules and the protocol that is in place from the Premier League and set a positive standard to the millions of people watching across the world."
He added of the handshake: "We'll go ahead with it, yes."
