The Bulgarian was widely reported to have flown to Germany earlier this week, which led to speculation linking him with Leverkusen and Bayern Munich.
Now Leverkusen, who sold Berbatov to Tottenham in July 2006, have confirmed that they have no interest in bringing the striker back to the BayArena.
"I have been reading that Dimitar (Berbatov) would have flown over to Frankfurt and that we would be in for him there to negotiate a transfer. That was interesting news to me," Bayer Leverkusen executive Wolfgang Holzhauser told Express. "I am clearly out to never say never, but this is nothing we are looking forward to at this point in time.
"And from today's point of view I would also say we are not after him in the coming summer."
Speaking to the Russian media, his agent Emil Danchev also insisted that a move was not on the cards, after Anzhi Makhachkala and CSKA Moscow were also linked.
"Usually I don't comment on speculation from the press. They can say whatever they want," Danchev told Sovetski Sport.
"But the only thing that I can say is that at this moment the eventual transfer of Berbatov to another club is not on the agenda."