Red Sox INF Holt leaves with left hamstring tightness
Holt singled with one out in the second, advanced on Christian Vázquez's single and was cut down at the plate by left fielder Lourdes Gurriel Jr.'s throw trying to score on Jackie Bradley Jr.'s single.
Holt was replaced by Eduardo Núñez in the top of the fourth.
Holt has been Boston's hottest hitter since returning from the injured list on May 27, leading the team with a .357 average coming into Sunday.
He went on the IL on April 7 with a scratched right cornea and also on injured his right shoulder while on a rehab assignment.