George Beamon scored 21 points and Manhattan scored the final 17 points of the game to defeat Pennsylvania 59-54 on Wednesday night.
The Jaspers (2-0) trailed 54-42 with 7:48 remaining in the game before outscoring the Quakers 17-0 behind Beamon, who scored nine points in the run.
Beamon, who added nine rebounds, converted a three-point play to begin the run and cut Penn's lead to 54-45. His two free throws gave the Jaspers a 55-54 advantage with 1:15 remaining.
Penn (1-1) had led from midway through the first half and took a 54-42 lead - its biggest of the game - on Jack Eggleston's 3-pointer with 7:48 left.
Kidani Brutus scored 14 points and had 10 rebounds, and Rhamel Brown added 10 points for Manhattan.
Eggleston finished with 22 points and 11 rebounds, and Zack Rosen scored 14 points for Penn.