Barnsley are expected to be without midfielder Kelvin Etuhu when they face Wolves at Oakwell on Tuesday.
The former Manchester City youngster sustained a broken nose when he clashed heads with Adam Chicksen during the Tykes' 3-1 win over MK Dons in the fifth round of the FA Cup on Saturday.
Etuhu, who received treatment on the pitch for seven minutes before being substituted off, looks set to miss out on Tuesday, with Jim O'Brien his most likely replacement.
Otherwise, manager David Flitcroft is unlikely to make any further changes to his starting XI.
Striker Jason Scotland, who has stepped off the substitutes' bench in his side's last four matches, is in contention to make his first start for the club.
Stephen Ward is expected to shrug off illness and keep his place for Wolves, with new loan capture Jack Robinson set to take his place on the bench.
Left-back Ward played the full 90 minutes on Saturday as Wolves drew 0-0 at Derby but duly missed training on Monday struggling with illness.
On the same day, manager Dean Saunders was also putting the finishing touches to Robinson's loan move from Liverpool although the player, 19, is not expected to replace Ward, who should recover in time to start at Oakwell.
Kevin Foley and Jamie O'Hara are both battling to overcome knocks picked up at the weekend but also expect to be fit.
There is further good news for boss Dean Saunders, who is still awaiting his first win in charge of Wanderers, as centre-back Roger Johnson returns from his three-match suspension.
Roberto Martinez's side are yet to record their first league win in 2013 and are currently second-bottom in the overall standings, three points from safety.
The FA Cup has provided something of a tonic for the Wigan, who on Sunday booked their place in the quarter-finals for the first time in 26 years thanks to a 4-1 win over Huddersfield.
Victories over Macclesfield, Bournemouth and now the Terriers in the cup competition have been the only successes this calendar year for Wigan, and Stam wants to harness that spirit when they return to Premier League action against Reading at the weekend.
"It was great to win the game in the way we did and we're all really pleased to be through to the next round," Stam told Wigan's website.
"The atmosphere we have started to create in the dressing room is really good but in the end it all depends on the result and we have to make sure that we get the results to keep everybody's spirits high.
"We have to win games and when we do what we did against Huddersfield it really gives us the confidence to carry into the next game.
"We all want to take this performance into the next league game against Reading which is a higher level but if we play to our own strengths then it gives us a great chance of collecting three points.
"Now it's all about being ready for next weekend against Reading and continuing this winning feeling."
Wigan will play either Oldham or Everton in the last eight of the FA Cup, with their opponents to be decided at the end of the month in a fifth-round replay.
"We're coming closer and closer to Wembley now but we'll have to wait and see whether we get Oldham or Everton and then prepare from there," Stam said.
"It's the second time in a row now that we don't know who we are playing yet but it doesn't matter because we have to be ready for anything.
"When the time comes we just have to work hard like we did in the build-up to the fifth round and then who knows how far we could go."
Tranmere will have to make do without defender Danny Holmes.
Holmes suffered a head injury during Friday night's televised 1-0 defeat to Shrewsbury and is set to miss out.
Liam Palmer received a straight red card in the first half of that match and begins a three-game ban, possibly paving the way for fellow Sheffield Wednesday loanee Paul Corry to make his full debut in midfield having featured as a second-half substitute against the Shrews.
Adam McGurk sustained a hamstring injury in training on Monday morning and fellow forward Jean-Louis Akpa Akpro remains sidelined as he completes a three-match suspension.
Rovers have lost four of their last five matches but could return to the automatic promotion places in League One with victory over Swindon, who thrashed them 5-0 at the County Ground in December.
Skipper James Wallace remains out amid fears the former Everton midfielder's knee injury could force a premature end to his season.
Swindon will be without the services of Joe Devera once again.
The former Barnet left-back's comeback from his initial problem lasted just 35 minutes at Leyton Orient last month but, after a fortnight of rehabilitation, he is edging closer to a first-team return, although this match comes too soon for the 26-year-old.
Defender Troy Archibald-Henville remains out with his knee injury and Alan McCormack is ill, but manager Paolo Di Canio has no fresh injury worries as his Robins look to extend their unbeaten run to 11 games.
That impressive streak has moved Swindon to within two points of second-placed Sheffield United ahead of the trip to Prenton Park.
The teams last met back in December when the Robins ran out 5-0 winners at The County Ground.
