Chilean Hector Mancilla and Antonio Naelson scored as reigning champion Toluca defeated America 2-1 on Sunday in the eighth round of the Mexican Apertura championship.
Mancilla put the home side ahead on the 24th minute, but Matias Vuoso got the equalizer in the 48th before Naelson scored the winning goal with a free kick.
The Red Devils extended to seven their streak of consecutive games without a loss and now have 13 points to lead Group 2.
Also on Sunday, Brazilian striker Itamar Batista scored a hat-trick as Tigres defeated Puebla 3-1.
"We are very happy with the result and we are very satisfied," Mancilla said. "We took the group lead and defeated a very tough rival, now we have to be ready for the next round."
America lost for the first time since the first week of the season and stayed on 12 points in Group 2.
In Puebla, Batista struck in the first half for the Tigres who leaped to 11 points, good for third place in Group 1.
The Brazilian recorded his first hat-trick in Mexico, where he has played since the Clausura 2008.
Argentine midfielder Gabriel Pereyra scored on a free kick in the 64th for Puebla, which lost its second in a row.
Cruz Azul is the overall leader in Mexico with 21 points, topping Group 3. Monterrey and Santos each have 18 to lead Group 1.
On Saturday, it was: Cruz Azul 3, Queretaro 0; San Luis 1, Pachuca 0; Santos 2, Necaxa 1; Monterrey 2, Atlas 0; Chivas 0, Jaguares 3; Atlante 2, Morelia 0.