West Brom goalkeeper Boaz Myhill will again provide cover for Ben Foster, who is recovering from groin surgery.
Albion are sweating over the fitness of defender Gareth McAuley, who suffered a hamstring injury playing for Northern Ireland against Azerbaijan, with Gabriel Tamas on stand-by to deputise.
But they are more hopeful midfielder Youssouf Mulumbu will be fit after a hamstring injury led to him withdrawing from midweek action with Congo. On-loan Chelsea striker Romelu Lukaku is ineligible to face his parent club.
Chelsea will once again be without the services of club captain John Terry after he picked up a knee injury during a meeting with Liverpool last weekend.
The centre-half made his return to Premier League action after serving a four-match ban in a 1-1 draw with the Reds, but is now facing up to another spell on the sidelines.
Ashley Cole, who has been nursing a hamstring problem, and Frank Lampard, who has a calf complaint, remain doubtful, while Di Matteo will be hoping that a midweek trip to the USA has not taken too much out of Brazilian duo Oscar and David Luiz.
Spireites manager Paul Cook could welcome back Tommy Lee and Marc Richards.
Highly-rated goalkeeper Lee (shoulder) and striker Richards (hamstring) have been sidelined since the start of September and are "very close" to making their comebacks.
When asked if the duo will be fit for Saturday, Cook, who has also been without on-loan defender Liam Cooper, midfielder Mark Allott (hamstring) and striker Luis Boa Morte (groin) in recent weeks, said: "Watch this space.
"We have to be careful what we say about injuries as last week we said Tommy Lee would be ready, so then all the fans get excited, but he wasn't.
"We obviously want everyone back as soon as possible. But what I will say is that we have had a number of players who have been out injured who are now training with the first team and they are very close."
Cook hinted that Brennan Dickenson would make his full debut against the Us. The teenage forward, who joined Chesterfield on loan from Brighton last week, marked his first appearance for the club from the bench with a goal during the 3-2 defeat at Bristol Rovers.
Alfie Potter has given Oxford boss Chris Wilder a boost ahead of their trip to Derbyshire by returning to training after a hamstring injury.
The 23-year-old, who has scored five times this season, missed the defeat at Dagenham and the draw with Torquay last weekend but is expected to make the trip north after making a full recovery.
Wilder handed defender Liam Davis his first start of the season against the Gulls after the full-back had been missing since May through injury.
Until their recent blip Oxford had only lost two of their previous eight games, a spell which included a 5-0 thrashing of Accrington and a 3-1 win away to Wycombe, but one point from their last two games means they are 17th in the standings.
