Midfielder Paul Green is hoping to return to the Leeds squad ahead of Blackpool's visit to Elland Road on Wednesday night.
Green was denied the chance to play against Premier League champions Manchester City in the FA Cup fifth round on Sunday after suffering an ankle injury in the build-up to the game.
"Greeny went over his ankle," said under-pressure Leeds manager Neil Warnock, who reported no new problems following the defeat at the Etihad Stadium.
"He was desperately disappointed. He asked if he could have a late fitness test, but I said no. I would rather him be fit for Wednesday, and I would think we will have him back for then."
Warnock also revealed he made his half-time substitutions against City with Wednesday's game in mind. Both Michael Brown and Ross McCormack were replaced at the break in Manchester.
"I wanted to keep Browny and Ross for Wednesday," Warnock said. "Browny is doing brilliant at the minute and he's played a lot of football, and Ross is obviously a big player for us and I want him for Wednesday."
Paddy Kenny is likely to return in goal against Blackpool after Jamie Ashdown was once again used in a cup competition.
Tom Ince is expected to return to the starting line-up when his father takes charge of Blackpool for the first time.
Paul Ince was confirmed as Michael Appleton's successor in the Bloomfield Road hot seat on Monday after agreeing a one-year rolling contract.
Ince Jnr has been in sensational form for the Seasiders this term, netting 17 goals in 33 appearances.
But he only featured from the bench during Saturday's 1-0 defeat to Ipswich, coming after his return from an appeal at UEFA's headquarters in Switzerland against a one-match international ban dating back to England Under-21s' fiery European Championship play-off against Serbia in October.
Matt Gilks returned from suspension at Portman Road and will keep his place in goal.
Elsewhere, left-back Bob Harris is still struggling with a toe injury and fellow defender Ian Evatt (knee) is set to miss the rest of the season.
Grayson's three previous managerial assignments at Blackpool, Leeds and Huddersfield have all seen him plot routes out of the third tier and he holds similar ambitions for his time at Deepdale.
"That's why I've come here," he said. "I've not come to the football club to have a relaxing time. I'm passionate about achieving things and positive about achieving things.
"I've got the experience of League One but I've also got the experience of taking Leeds to seventh in the Championship and, when I left there, three points off the play-offs.
"I've got the experience of many different divisions but it would be nice to try and get another promotion on my CV."
But with North End languishing in the bottom half of the division following a torrid season under Graham Westley, Grayson accepts his short-term goal is to extinguish any lingering relegation fears.
"We're eight points off that bottom four and we've got to have on eye on it, I suppose, but my glass is always half-full.
"When you're in the bottom half of the division you've got to be aware that a run can take you into that bottom four.
"So we're realistic about what might happen but we're more positive on trying to get up the other end because that's what the players should be doing based on what we've got here."
