Gabriel Agbonlahor has been ruled out of England's friendly with Spain at Wembley on Saturday with a hamstring injury.
The Aston Villa man was hoping to win his fourth cap but suffered an injury in training on Thursday.
While the damage is not thought to be serious, it was decided to send Agbonlahor back to Villa for further treatment, ending his chances of facing the world and European champions.
"Gabriel Agbonlahor has this morning returned to Aston Villa," confirmed the FA on Friday.
"Having been assessed by the England medical staff, Agbonlahor is unable to train at Wembley and has returned to his club."
The England players and coaching staff held a two minutes' silence in the Wembley centre circle in honour of Remembrance Day before undertaking their final training session ahead of Saturday's game.
Skipper John Terry stood alongside head coach Fabio Capello while Frank Lampard, who will wear the armband against Spain as Terry has not been selected, was next to Under-21 coach Stuart Pearce.
