Midfielders Andres Iniesta and Xavi Hernandez have added their names to Barcelona's list of key injured players, ruling them out of Friday's Copa del Rey opener at third-tier Cartagena.
Barcelona says both players will rest for precaution after they noted pain in leg muscles.
Barcelona, which was already without injured forward Lionel Messi, goalkeeper Victor Valdes and defender Dani Alves, has recovered defender Jordi Alba. Two muscle injuries had sidelined Alba since mid-September.
Coach Gerardo Martino left Gerard Pique off his squad.
Barcelona has lost its last two games, but it still shares the joint lead of the Spanish league with Atletico Madrid.
Barcelona will play the return leg of its round-of-32 tie with Cartagena at Camp Nou in two weeks.
