Following the resounding win over the Danish club – in which Cristiano Ronaldo and Angel Di Maria scored two goals apiece – Ancelotti moved to allay fears over Bale’s condition, promising the winger would use the upcoming international break to reach a "good physical condition".
But on Thursday morning, Wales manager Chris Coleman called Bale into his squad to face Macedonia and Belgium in World Cup qualifiers on October 11 and 15.
Dragons boss Coleman did, however, confirm the player would not actually feature in either game.
Ancelotti had told Real’s official website: "We are not worried, he has not played because of muscle fatigue and it is nothing special.
"He is waiting to recover his optimal fitness. He was not at 100 per cent but he will get there because he has 15 days of rest and then will be ready.
"He is not happy and that is normal, he wants to play and is eager but it is not a problem. Now we have time to get him into a good physical condition and avoid injuries."
Bale moved to the Santiago Bernabeu for a world-record fee in September after missing much of Spurs’ pre-season campaign through injury and he has only started once for Los Blancos – scoring on his debut in a 2-2 draw at Villarreal.
A training update posted on Real’s website on Thursday morning said Bale was continuing his recovery alongside midfielder Xabi Alonso, who has not played this season due to a broken foot.