Worley's spring ERA rose to 13.50 and didn't get through the third inning.
Among eight Twins out of minor-league options, Worley is battling fellow right-handers Sam Deduno and Kyle Gibson and left-hander Scott Diamond for the final rotation spot.
"First inning I wasn't able to get the ball down in the zone, and they hit the ball pretty much everywhere," Worley said. "Third inning, we thought we had everything on the right path, and everything unraveled again."
Worley, acquired from Philadelphia two offseasons ago in a trade that cost the Twins starting outfielder Ben Revere, fell behind on the first pitch to nine of 19 batters.
Shortstop Pedro Florimon made his Grapefruit League debut just over one month after undergoing an appendectomy. Florimon had played a total of eight innings on the minor-league side, then had his scheduled debut on Monday rained out in Sarasota.