Tulowitzki was running out a groundball to shortstop in the top of the fourth inning when he grabbed his left leg halfway down the first-base line. He was thrown out easily and gingerly made his way to the dugout before being replaced by Josh Rutledge.
The 29-year-old Tulowitzki is in the middle of a career season. He began play Saturday leading the major leagues with a .342 batting average. His 71 runs are also tops in the majors.
"Nothing serious," Tulowitzki said after Rockies' 3-2, 11-inning loss. "We'll see how I wake up (Sunday). The good news is I don't think I tore anything or did anything to where I'm going to miss an extended period of time."