Wade is out a second straight night with the injury. He'll sit out Saturday night's game at the Bradley Center, where he played in college for Marquette.
Besides Wade, Miami will be without Mario Chalmers, Ray Allen and Greg Oden. The quartet also missed Friday night's game against Detroit -- though it mattered little after LeBron James notched a triple-double in leading the Heat to a 110-78 victory.
Wade has played in 51 of 72 games this season, having missed action for ailments including a sore knee, a sore ankle and back spasms.
Wade has said this hamstring injury isn't as serious as one he had in the past.